- Clarence as Mario
- Sumo as Luigi
- Jeff as Yoshi
- Cooter as Waluigi
- Seabass as Wario
- Belson as Bowser Jr.
- Malakevin as Kamek
- Lil' Grandpa (From "The Break Up") as Toadsworth
- Amy Gillis as Princess Peach
- Chelsea Keezheekoni as Princess Daisy
- Nature Kate as Princess Rosalina
- Amy Shtuzger as Candy Kong
- Joshua Maverick as Bowser
- Unnamed Hooded Kid with Pimples (From the episode "Spooky Boo") as Fawful
- Malessica as Birdo
- Emilio as Baby Bowser
- Glary Keezheekoni as Midbus
- Courtlin as Pauline
- Nathan as Donkey Kong
- Percy as Diddy Kong
- Mavis as Toadette
- Brady as Toad
- Ms. Lofton as Cackletta
- Game Show Host Guy (From "Game Show") as Antasma
- Tinia as Kammy Koopa
